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The date on which the opened vial will be considered expired should be calculated (up to 28 days after the vial opening) and mentioned on the label

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Elizabeth Rebello of the University of Texas MD Anderson Center

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The samples were then incubated with the equilibration buffer provided in the TUNEL assay kit at room temperature for 10 min, followed by incubation with the reaction mixture containing terminal deoxynucleotidyl transferase and labeled dUTP